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STOCK En1tm2(cre)Wrst/J
Cat. No.: CEMM-07250082
These En1Cki (or En1Cre) mutant mice harbor a Cre recombinase (cre) "knock-in" allele that also abolishes endogenous gene function and may be useful for Cre-lox applications studying engrailed protein function such as deleting genes in spinal cord V1 interneurons, the embryonic mesencephalon and rhombomere 1 by E9, as well as in the ventral ectoderm of the limbs, in a subset of somite cells, and some mesoderm-derived tissues.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
En1tm2(cre)Wrst
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| Allele Name |
targeted mutation 2
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| Allele Attributes |
Recombinase-expressing; Null/Knockout
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| Gene Symbol |
En1
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| Gene Name |
engrailed 1
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| Chromosome |
1
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| Expressed Genes |
Cre, Cre recombinase, bacteriophage P1
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| MGI Accession ID | show more close |
| Site of Expression |
Spinal cord V1 interneurons, the embryonic mesencephalon and rhombomere 1 by E9, as well as in the ventral ectoderm of the limbs, in a subset of somite cells, and some mesoderm-derived tissues.
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| Strain of Origin |
(129X1/SvJ x 129S1/Sv)F1-Kitl+
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| Molecular Note |
A Cre recombinase gene cassette was inserted into the En1 locus. A loxP flanked neomycin cassette is removed in vivo via a Cre-mediated recombination event.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
Wild-type from the colony
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| Breeding Considerations |
When maintaining a live colony, heterozygous mice may be bred to wildtype siblings. Homozygotes die at birth. For Cre-lox experiments, researchers are urged to monitor their Cre-floxed double mutant mice for any undesirable germline deletion of the floxed allele (e.g. genotyping tail/ear samples). Researchers may consider also maintaining an En1Cki colony separate from any floxed colonies. See Detailed Description for more details.
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For Research Use Only.
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