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B6.Cg-Gt(ROSA)26Sortm2(CAG-rtTA3, -mKate2)Slowe/J
Cat. No.: CEMM-07250744
Common Name: Rosa26-CAGs-LSL-RIK knock-in
CAGs-LSL-RIK (or Rosa26-CAGs-LSL-RIK) knock-in has a CAG promoter and loxP-flanked STOP cassette upstream of the third-generation reverse tetracycline-regulated transactivator gene (rtTA3), IRES sequence and the monomeric far-red fluorescent protein mKate2, all inserted into the Gt(ROSA)26Sor locus. These mice are a Cre recombinase-dependent, Tet-On and fluorescent tool strain - following removal of the floxed-STOP cassette, they allow dox-conditional expression of TRE promoter-driven target genes in conjunction with mKate2 fluorescence.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm2(CAG-rtTA3,-mKate2)Slowe
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| Allele Name |
targeted mutation 2
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Transactivator
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
rtTA, reverse tetracycline-controlled transactivator, E. coli
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| MGI Accession ID | show more close |
| Site of Expression |
When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will express rtTA3 in Cre-expressing tissues with subsequent expression of RFP (mKate2) under control of a tetracycline-responsive promoter element (TRE; or tetO) that can be induced/regulated by tetracycline or its analog doxycycline (dox).
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| Strain of Origin |
(C57BL/6J x 129S4/SvJae)F1
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| Molecular Note |
The targeting construct introduced into the locus a CAG promoter, floxed stop cassette, reverse tetracycline transactivator 3 (rtTA3), IRES and a far red reporter gene (mKate2).
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6NJ
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| Breeding Considerations |
Mice heterozygous or homozygous for the Rosa26-CAGs-LSL-RIK knock-in allele are viable and fertile with no reported gross physical or behavioral abnormalities. When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6NJ inbred mice. Alternatively, homozygous mice may be bred together.
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For Research Use Only.
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