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STOCK Stag2tm1c(EUCOMM)Wtsi/J
Cat. No.: CEMM-07250833
Common Name: Stag2flox
The "conditional ready" (floxed) Stag2tm1c(EUCOMM)Wtsi allele (Stag2flox) has loxP sites flanking exon 7. Exposure to Cre recombinase creates the knock-out allele. These mice may be useful in studying the cohesin complex and STAG2 loss-of-function mutations in diseases such as cancer.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Stag2tm1c(EUCOMM)Wtsi
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| Allele Name |
targeted mutation 1c
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| Allele Attributes |
Conditional ready (e.g. floxed); No functional change
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| Gene Symbol |
Stag2
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| Gene Name |
stromal antigen 2
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| Chromosome |
X
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| MGI Accession ID | show more close |
| Strain of Origin |
C57BL/6N
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| Molecular Note |
The L1L2_gt0 cassette was inserted upstream of the critical exon (exon 7) with insertion at bp position ChrX:42,222,777 [Build GRCm38]. The L1L2_gt0 cassette is composed of an FRT site, a mouse En2 intron/splice-acceptor/exon (En2 SA), a self-cleaving 2A peptide, the beta-galactosidase gene (lacZ), a second self-cleaving 2A peptide, the neomycin-resistance gene (neo), an SV40 polyadenylation signal (SV40pA), a second FRT site and a loxP site. A second loxP site was inserted downstream of exon 7 at bp position ChrX:42,223,727 [Build GRCm38]; therefore flanking exon 7 with loxP sites. Flp-mediated recombination removed the FRT flanked neo cassette leaving exon 7 floxed.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
The floxed allele is on the X chromosome. Homozygous females (Stag2flox/flox) and hemizygous males (Stag2flox/Y) are viable and fertile, with no reported gross phenotypic abnormalities or breeding problems. When maintaining a live colony, homozygous females may be bred to hemizygous males.
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| Breeding Strategy |
Homozygote x Hemizygote
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For Research Use Only.
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