STOCK Insm1^tm1.1Mgn/Mmjax

Insm1^tm1.1Mgn

targeted mutation 1.1

Recombinase-expressing; Reporter

Insm1

insulinoma-associated 1

2

GFP/Cre, Green Fluorescent Protein

5576020

A GFP/Cre fusion cassette replaces the Insm1 gene, which is normally expressed in pancreatic endocrine progenitor cells beginning at E9.5, and also in neural and olfactory epithelium precursor cells.

129S6/SvEvTac

Using recombinase mediated cassette exchange (RMCE) a 532 bp fragment containing a GFP-Cre fusion protein replaced the entire coding region of the gene. The donor construct includes a FRT flanked pgk-hygromycin immediately downstream of the GFP-Cre fusion sequence. The FRT flanked pgk-hygromycin was removed by FLPe mediated recombination. Western blot analysis confirmed absence of protein in homozygotes. Green fluorescence is seen in panCreatic pre-endocrine cells and in islet cells after birth, in the developing central nervous system between E9.5 and E18.5, and also in the peripheral nervous system and gut endocrine cells.

While maintaining a live colony, these mice are bred as heterozygotes. Mice homozygous for the mutation are not viable.