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C57BL/6J-Tg(CAG-lacZ, -FUS*R521G, -EGFP)673Gyu/J
Cat. No.: CEMM-07250649
Common Name: CAG-Z-FUSR521G-IRES-EGFP transgenic line 673 [CAG-Z-FUSR521G (673)]
The CAG-Z-FUSR521G-IRES-EGFP transgenic line 673 [or CAG-Z-FUSR521G (673)] has widespread expression of lacZ, that is replaced by expression of the human fused in sarcoma R521G mutant isoform (huFUSR521G) and EGFP fluorescence following exposure to Cre recombinase. The Cre recombinase-inducible expression of huFUSR521G, along with similarly designed transgenic mice harboring the human wildtype FUS, is useful in studying FUS-linked juvenile amyotrophic lateral sclerosis (ALS; Lou Gehrig's Disease), and the neurodegenerative disorder frontotemporal lobar degeneration (FTLD).
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Tg(CAG-lacZ,-FUS*R521G,-EGFP)673Gyu
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| Allele Name |
transgene insertion 673
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Inserted expressed sequence; Humanized sequence
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| Gene Symbol |
Tg(CAG-lacZ,-FUS*R521G,-EGFP)673Gyu
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| Gene Name |
transgene insertion 673
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| Chromosome |
UN
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| Expressed Genes |
FUS, fused in sarcoma, human; GFP, Green Fluorescent Protein; lacZ, beta-galactosidase, E. coli
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| MGI Accession ID | show more close |
| Site of Expression |
Prior to Cre recombination LacZ is widely expressed; the human fused in sarcoma R521G mutant isoform and EGFP are expressed only after Cre recombination in Cre-expressing tissues.
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| Strain of Origin |
C57BL/6J
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| Molecular Note |
This transgene consists of the cytomegalovirus immediate early enhancer-chicken beta-actin hybrid (CAG) promoter, a floxed lacZ gene, human FUS cDNA with the R521C mutation, IRES, and EGFP coding sequences. Cre-excision of the loxP flanked lacZ DNA allows for translation of FUS and EGFP. Two lines were generated: 673, and 682. Line 682 was chosen as a representative line.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
When maintaining a live colony, transgenic mice may be bred with wildtype (non-carrier) mice from the colony or with C57BL/6J inbred mice. it has not been attempted to make this strain homozygous.
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| Breeding Strategy |
Noncarrier x Hemizygote; Hemizygote x Noncarrier
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For Research Use Only.
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