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STOCK Pou4f2tm1(cre)Bnt/J
Cat. No.: CEMM-07250798
Common Name: Pou4f2Cre
This knock-in/knock-out strain expresses cre recombinase from the Pou4f2 promoter. Homozygosity results in a reduction in retinal ganglion cells (RGCs). Cre expression is observed in the retina, brain, intestines, heart, and gonads. Males carrying both this Pou4f2Cre allele and a floxed region of interest produce progeny that exhibit a widespread pattern of germline expression in all tissues tested.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Pou4f2tm1(cre)Bnt
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Recombinase-expressing
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| Gene Symbol |
Pou4f2
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| Gene Name |
POU domain
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| Chromosome |
8
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| Expressed Genes |
Cre, Cre recombinase, bacteriophage P1
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| MGI Accession ID | show more close |
| Site of Expression |
When Pou4f2Cre mice are crossed with animals carrying a loxP-flanked sequence of interest, Cre-mediated excision of the floxed region occurs in the retina, brain (optic tract, hippocampus, cortex), intestines, heart, and gonads (ovary and testes).
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| Strain of Origin |
Not Specified
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| Molecular Note |
An internal ribosome entry site (IRES)-Cre was fused to exon 1, disrupting the mouse Pou4f2 gene. A neomcyin cassette, in reverse transcriptional orientation, was placed downstream.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
Wild-type from the colony
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| Breeding Considerations |
Heterozygotes are viable and fertile. Homozygotes are viable, but not fertile.
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| Breeding Strategy |
Wild-type x Heterozygote; Heterozygote x Wild-type
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For Research Use Only.
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