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STOCK Notch1tm4(cre)Rko/J
Cat. No.: CEMM-07250632
Common Name: N1IP::CreHI
The high-activity Notch1 activation-dependent reporter knock-in allele, N1IP::CreHI, has the endogenous Notch1 intracellular domain (NICD1) replaced with a nuclear-localized Cre recombinase. Compared to N1IP::CreLO, this N1IP::CreHI allele has significantly improved sensitivity with five- to ten-fold increase in Cre activity. This improved sensitivity allows users to label cells experiencing moderate-to-lower Notch activation thresholds; including most cells/tissues known to utilize Notch1 during their development and/or maintenance.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Notch1tm4(cre)Rko
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| Allele Name |
targeted mutation 4
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| Allele Attributes |
Recombinase-expressing
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| Gene Symbol |
Notch1
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| Gene Name |
notch 1
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| Chromosome |
2
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| Expressed Genes |
Cre, Cre recombinase, bacteriophage P1
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| MGI Accession ID | show more close |
| Site of Expression |
Endothelial cells.
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| Strain of Origin |
129X1/SvJ
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| Molecular Note |
The Notch1 intracellular domain (IC) is replaced by the Cre recombinase sequence and includes the SV40 3' UTR polyA signal while introducing a single nucleotide variation (SNV) at C5178A to facilitate genotyping. This allele does not have the 6xMyc tag present in the Notch1tm3(Cre)Rko allele, and displays enhanced activity relative to that allele.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
Wild-type from the colony
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| Breeding Considerations |
When maintaining a live colony, heterozygous mice are bred to wildtype mice from the colony. Homozygotes die in utero.
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For Research Use Only.
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