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STOCK Notch1tm2Rko Notch2tm3Grid Gt(ROSA)26Sortm9(CAG-tdTomato)Hze Tg(KRT5-cre/ERT2)2Ipc/JeswJ
Cat. No.: CEMM-07250830
Common Name: K5CreER; N1fl; N2fl; Ai9 [or K5-fl(N1/N2)-Ai9]
K5CreER;N1fl;N2fl;Ai9 mice (or K5-fl(N1/N2)-Ai9) are quadruple mutant animals with tamoxifen-inducible Cre recombinase expression directed to basal epithelial cell populations - resulting in Notch1/Notch2 knock-out and robust tdTomato fluorescence. These mice allow inducible genetic manipulations for studying Notch pathway disruption in epithelial tissues (e.g., epidermis, salivary gland, digestive tract, peripheral olfactory system) as well as UV-light skin damage and cancer biomarkers.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm9(CAG-tdTomato)Hze
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| Allele Name |
targeted mutation 9
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
RFP, Red Fluorescent Protein, coral
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| MGI Accession ID | show more close |
| Site of Expression |
A loxP-flanked STOP cassette is designed to prevent transcription of the red fluorescent protein variant tdTomato. Upon exposure to Cre recombinase, robust tdTomato fluorescence may be observed. Importantly, the donating investigator reports that very low levels of tdTomato expression may be present prior to introduction of Cre recombinase - but the tdTomato expression levels after Cre recombination are significantly greater than those baseline levels.
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| Strain of Origin |
(129S6/SvEvTac x C57BL/6NCrl)F1
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| Molecular Note |
The Rosa-CAG-LSL-tdTomato-WPRE targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), tdTomato sequence (a non-oligomerizing DsRed fluorescent protein variant with a 12 residue linker fusing two copies of the protein (tandem dimer)), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a polyA signal, and an attB/attP-flanked PGK-FRT Neo-polyA cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
When maintaining a live colony, mice homozygous for the three targeted mutations and hemizygous for the transgene (K5CreER;N1fl/fl;N2fl/fl;Ai9lsl-tdT/lsl-tdT) may be bred with mice homozygous for all three targeted mutations and wildtype (non-carrier) for the transgene (N1fl/fl;N2fl/fl;Ai9lsl-tdT/lsl-tdT). The donating investigator reports that mice homozygous for the K5CreERT2 transgene (as identified by test cross/progeny ratios) are viable and fertile.
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For Research Use Only.
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