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STOCK Igs6tm1.1(ACTB-tdTomato, -EFGP)Luo/J
Cat. No.: CEMM-07250453
Common Name: MADM-12TG (John12TG)
The MADM-12TG (Igs6TG) allele has the CMV enhancer/chicken beta-actin core promoter, the N-terminal portion of tdTomato, a beta-globin intronic sequence (containing an frt site and loxP site), and the C-terminal portion of mut4-EGFP all inserted into the Igs6 locus on chromosome 12 (~1.71 cM; ~16 kbp downstream of exon 1 of the Rab10 gene). These MADM-12TG mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to MADM-12GT mice harboring a reciprocal mutation at the same locus. This MADM system allows Cre recombinase-induced fluorescent labeling of daughter cells to ascertain lineal relationships and pleiotropic gene function in multicellular organisms. These mice may also be useful in studies of cell differentiation, mitosis, and imprinting.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Igs6tm1.1(ACTB-tdTomato,-EFGP)Luo
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| Allele Name |
targeted mutation 1.1
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| Allele Attributes |
Reporter; Null/Knockout
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| Gene Symbol |
Igs6
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| Gene Name |
intergenic site 6
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| Chromosome |
12
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| Expressed Genes |
GFP, Green Fluorescent Protein; RFP, Red Fluorescent Protein, coral
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| MGI Accession ID | show more close |
| Site of Expression |
EGFP and tdTomato will be expressed in cells/tissues where promoters driving Cre recombinase and Flp recombinase are expressed.
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| Strain of Origin |
(129X1/SvJ x 129S1/Sv)F1-Kitl+
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| Molecular Note |
The TG MADM targeting construct was designed with a CMV enhancer/chicken beta-actin core promoter (pCA), the N-terminal portion of a red fluorescent protein (tdTomato; aa 1-3), a beta-globin intronic sequence containing an FRT site and loxP-flanked neomycin resistance gene, the C-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP; aa 274-724), and an SV40 T-antigen poly(A) signal. This entire TG MADM construct was inserted into the Iis6 locus located about 16 kb downstream of exon 1 of the Rab10 gene at 1.71 cM. Cre-mediated recombination removed the neo cassette.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
B6129SF2/J
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| Breeding Considerations |
When maintaining a live colony, homozygous mice may be bred together.
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For Research Use Only.
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