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STOCK HhatTg(TFAP2A-cre)1Will/J
Cat. No.: CEMM-07250497
Common Name: CrefaceT
These Creface transgenic mice have a cre-recombinase sequence driven by the human transcription factor AP-2, alpha promoter/enhancer elements, and a polyadenylation sequence followed by parts of exons 5-6 of Tfap2a, encoding the frontonasal prominence (FNP) and limb enhancer elements. This transgene integrated into intron 9 of the hedgehog acyltransferase (Hhat) gene, abolishing Hhat gene function. These mice may be useful for studying craniofacial development.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
HhatTg(TFAP2A-cre)1Will
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| Allele Name |
transgene insertion 1
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| Allele Attributes |
Recombinase-expressing; Null/Knockout
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| Gene Symbol |
Hhat
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| Gene Name |
hedgehog acyltransferase
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| Chromosome |
1
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| Expressed Genes |
Cre, Cre recombinase, bacteriophage P1
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| MGI Accession ID | show more close |
| Site of Expression |
Expression of Cre recombinase was detected in the embryonic face and limb mesenchyme.
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| Strain of Origin |
Not Specified
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| Molecular Note |
This transgenic construct consisted of 200 bp of human TFAP2A (TCFAP2A) promoter sequence (encoding a specific enhancer element) followed by NLS-Cre, an SV40 polyA signal, and a 1.8 kb TFAP2A genomic fragment extending from within exon 5 through a portion of exon 6. The human intron 5 sequence includes the frontonasal prominence- and limb-specific enhancer. Expression of Cre recombinase was detected in the embryonic face and limb mesenchyme. New research has determined that the transgene inserted into intron 9 of the Hhat locus. This insertion Creates a non-functional allele.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
FVB/NJ
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| Breeding Considerations |
When maintaining a live colony, heterozygous mice may be bred to wildtype (non-carrier) mice from the colony. Homozygous mice are embryonic lethal.
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For Research Use Only.
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