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STOCK Igs5tm2.1(ACTB-EGFP, -tdTomato)Luo/J

Cat. No.: CEMM-07250450

Common Name: MADM-7GT (Hipp7GT)
The MADM-7GT (Igs5GT) allele has the CMV enhancer/chicken beta-actin core promoter, the N-terminal portion of mut4-EGFP, a beta-globin intronic sequence (containing frt and lox sites), and the MYC-tagged C-terminal portion of tdTomato all inserted into the Igs5 locus on chromosome 7 (~2.13 cM; ~0.7 kbp downstream of exon 5 of the Rps9 gene). These MADM-7GT mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to MADM-7TG mice harboring a reciprocal mutation at the same locus. This MADM system allows Cre recombinase-induced fluorescent labeling of daughter cells to ascertain lineal relationships and pleiotropic gene function in multicellular organisms. These mice may also be useful in studies of cell differentiation, mitosis, and imprinting.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Igs5tm2.1(ACTB-EGFP,-tdTomato)Luo
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Allele Name
targeted mutation 2.1
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Allele Attributes
Conditional ready (e.g. floxed); No functional change
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Gene Symbol
Igs5
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Gene Name
intergenic site 5
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Chromosome
7
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Expressed Genes
RFP, Red Fluorescent Protein, coral; GFP, Green Fluorescent Protein
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MGI Accession ID
5470395
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Site of Expression
EGFP and tdTomato will be expressed in cells/tissues where promoters driving Cre recombinase and Flp recombinase are expressed.
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Strain of Origin
(129X1/SvJ x 129S1/Sv)F1-Kitl+
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Molecular Note
The GT MADM targeting construct was designed with a CMV enhancer/chicken beta-actin core promoter (pCA), the N-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP; aa 1-273), a beta-globin intronic sequence containing an FRT site and loxP-flanked neomycin resistance gene, the C-terminal portion of a red fluorescent protein (tdTomato; aa 4-1544) tagged with three copies of the Myc epitope at its C-terminus, and an SV40 T-antigen poly(A) signal. This entire GT MADM construct was inserted into the Iis5 locus locate at about 0.7 kb downstream of exon 5 of the Rps9 gene at 2.13cM. Mice were bred to Hprt-Cre mice (on an undisclosed genetic background) and the floxed neomycin resistance gene was removed.
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HUSBANDRY
Suggested Controls
B6129SF2/J C57BL/6J
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Breeding Considerations
When maintaining a live colony, homozygous mice may be bred together.
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For Research Use Only.
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