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B6(Cg)-Dlx5tm1(cre/ERT2)Zjh/J
Cat. No.: CEMM-07250198
The Dlx5-CreERT2 knock-in allele in these mice both abolishes Dlx5 (distal-less homeobox 5) gene function and expresses a CreERT2 fusion protein from the Dlx5 promoter/enhancer elements.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Dlx5tm1(cre/ERT2)Zjh
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Recombinase-expressing; Inducible; Null/Knockout
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| Gene Symbol |
Dlx5
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| Gene Name |
distal-less homeobox 5
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| Chromosome |
6
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| Expressed Genes |
cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene
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| MGI Accession ID | show more close |
| Site of Expression |
Tamoxifen-inducible Cre recombinase activity is observed in the cortex.
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| Strain of Origin |
C57BL/6
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| Molecular Note |
A targeting vector was designed to insert a CreERT2 fusion gene (Cre-ERT2; Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), an SV40 polyA signal, and an FRT flanked neo cassette into the initiation codon of the Dlx5 (distal-less homeobox 5) gene. This construct was electroporated into C57BL/6 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with white C57BL/6 mice to originate the colony. Mutant mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background (N10)) to remove the neo selection cassette.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J Wild-type from the colony
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| Breeding Considerations |
When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings, or to C57BL/6J mice.
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For Research Use Only.
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