Online Inquiry
STOCK Gt(ROSA)26Sortm5(ACTB-tTA)Luo Igs7tm94.1(tetO-GCaMP6s)Hze/J
Cat. No.: CEMM-07250532
Common Name: Ai94(TITL-GCaMP6s)-D; ROSA26-ZtTA
Ai94(TITL-GCaMP6s)-D;Rosa26-ZtTA (or Ai94D;Rosa26-ZtTA) mice have a Cre/Tet-dependent, fluorescent calcium indicator GCaMP6s inserted into the Igs7 locus (TIGRE; an intergenic region on mouse chromosome 9 that allows reporter expression to be tightly regulated), as well as a Cre-dependent tetracycline-controlled transactivator protein (tTA) inserted into the Gt(ROSA)26Sor locus on chromosome 6. After removal of the floxed-STOP cassette by Cre recombinase, resulting mice have doxycycline-inducible/reversible expression of GCaMP6 slow variant calcium indicator (GCaMP6s; an ultrasensitive detector of single neuronal action potentials with slower decay and response kinetics). Following subsequent calcium binding (such as neuronal activation), increased EGFP fluorescence is observed in those cells.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Igs7tm94.1(tetO-GCaMP6s)Hze
show more
close
|
| Allele Name |
targeted mutation 94.1
show more
close
|
| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Inducible
show more
close
|
| Gene Symbol |
Igs7
show more
close
|
| Gene Name |
intergenic site 7
show more
close
|
| Chromosome |
9
show more
close
|
| Expressed Genes |
GCaMP, Genetically encoded calcium indicator
show more
close
|
| MGI Accession ID | show more close |
| Site of Expression |
Expression of fast variant calcium indicator (GCaMP6s) is under the control of tetO in cells/tissues where promoters driving Cre recombinase are expressed.
show more
close
|
| Strain of Origin |
(129S6/SvEvTac x C57BL/6NCrl)F1
show more
close
|
| Molecular Note |
The replacement vector was designed with (from 5' to 3') an FRT3 site, two copies of chicken beta-globin HS4 insulator element (to reduce reporter gene expression in absence of transactivators), a modified Tet response element (TRE or tetO), a floxed-STOP cassette (stop codons in all three reading frames linked to synthetic pA-hGHpA-PGKpA), the GCaMP6 slow variant calcium indicator coding sequence, a WPRE sequence (to enhance mRNA stability), a BGH polyA, two copies of chicken beta-globin HS4 insulator element, an AttB site, a PGK-5'hygro cassette, an RNA splice donor and a FRT5 site. The GCaMP6 slow variant calcium indicator (GCaMP6s) is an ultrasensitive detector of single neuronal action potentials with slow response kinetics and is an improved version of GCaMP5G. Embryonic stem cells previously targeted with FRT3::AttB::PGK-neoR-polyA::FRT5::splice acceptor::3'hygro cassette::SV40 polyA:AttP in TIGRE, were re-transfected with the pTRE-LSL-GCaMP6s replacement vector and Flp recombinase vector for recombinase-mediated cassette exchange (RCME).Chimeric mice were bred with PhiC31 Gt(ROSA)26Sortm3(phiC31*)Sor mice to remove the AttB/AttP-flanked PGK-hygromycin-SV40polyA cassette and replace it with the recombined AttB/AttP site (AttL).
show more
close
|
| HUSBANDRY | |
|---|---|
| Suggested Controls |
Wild-type from the colony
show more
close
|
| Breeding Considerations |
When maintaining a live colony, mice heterozygous for both the Ai94D targeted mutation (Igs7tm94.1(tetO-GCaMP6s)Hze) and also for the R26-ZtTA targeted mutation (Gt(ROSA)26Sortm5(ACTB-tTA)Luo) may be bred to wildtype mice from the colony or to C57BL/6J inbred mice. Alternatively, mice heterozygous or homozygous for Ai94D and heterozygous for R26-ZtTA may be bred to wildtype mice from the colony or to C57BL/6J inbred mice. The Ai94D donating investigator reports that mice homozygous for Ai94D are viable and fertile with no reported gross physical or behavioral abnormalities. The R26-ZtTA donating investigator reports that mice heterozygous for R26-ZtTA are more healthy and fertile than mice homozygous for R26-ZtTA.
show more
close
|
For Research Use Only.
Related Products