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STOCK Gt(ROSA)26Sortm6(ACTB-EGFP*, -tdTomato)Luo/J
Cat. No.: CEMM-07250375
Common Name: R26GT
The R26GT allele has a CMV enhancer/chicken beta-actin core promoter-driven "MADM GT" cassette (GFPN-terminus-intron-tdT3MycATG-less) inserted into the Gt(ROSA)26Sor locus on chromosome 6. Like the original MADM system, this "new MADM-6" system provides a tool to generate genetic mosaics in which an individual organism contains somatic cells of different genotypes. This allows Cre or FLP recombinase-induced fluorescent labeling of daughter cells to ascertain lineal relationships and pleiotropic gene function in multicellular organisms. These mice may also be useful in studies of cell differentiation and mitosis.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm6(ACTB-EGFP*,-tdTomato)Luo
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| Allele Name |
targeted mutation 6
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| Allele Attributes |
Conditional ready (e.g. floxed); No functional change
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| MGI Accession ID | show more close |
| Site of Expression |
These mice contain somatic cells of different genotypes which have mosaic EGFP and tdTomato expression in daughter cells When using the "new MADM-6" system.
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| Strain of Origin |
(129X1/SvJ x 129S1/Sv)F1-Kitl+
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| Molecular Note |
A targeting vector was Created with a CMV enhancer/chicken beta-actin core promoter (pCA) upstream of the "MADM GT" cassette. The "MADM GT" cassette used here (GFPN-terminus-intron-tdT3MycATG-less) was designed with the N-terminal portion of a mutant enhanced green fluorescent protein (mut4EGFP first exon; nucleotide 1-274), a beta-globin intronic sequence (containing a FRT flanked region of two lox site variants [lox2272 and lox5171] and then a loxP-flanked neomycin resistance gene), an ATG-less tdTomato sequence (nucleotides 3-1548 including stop codon) tagged with three copies of the Myc epitope at its C-terminus, and an SV40 T-antigen poly(A) signal. This entire construct was subcloned into the pROSA26-PA targeting vector to generate the final targeting construct pROSA26-GT. The pROSA26-GT construct was inserted into the Gt(ROSA)26Sor locus.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
Wild-type from the colony
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| Breeding Considerations |
Mice homozygous for the R26GT allele are viable with no gross behavioral or observable abnormalities. The donating investigator reports homozygous females have no observed fertility problems, but homozygous males can have reduced fertility. When maintaining a live colony, homozygous mice may be bred together.
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For Research Use Only.
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