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B6;129S-Igs7tm166(tetO-EGFP*, CAG-tTA2)Hze/XwyJ
Cat. No.: CEMM-07251019
Common Name: TIGRE-MORF (Ai166)
TIGRE-MORF(Ai166) is one of a set of a Cre reporter tool strains (also including MORF1 and MORF3) that use mononucleotide repeat frameshift (MORF) as a translation switch for cell labeling in vivo. TIGER-MORF (Ai166) mice express a cre-dependent tetracycline-activated EGFP fluorescent protein that is proceeded by a polyguanine repeat (G22) MORF switch. Exposure to Cre recombinase removes both STOP cassettes unblocking EGFP fluorescence and tTA2 expression and in the presence of spontaneous frameshift the EGFP reporter provides sparse and stochastic labeling of neural cells. In addition, reporter expression may be diminished/eliminated by doxycycline. TIGER-MORF(Ai166) can be used for high level direct fluorescent sparse labeling of Cre+ neuronal and glial cells.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Igs7tm166(tetO-EGFP*,CAG-tTA2)Hze
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| Allele Name |
targeted mutation 166
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Inducible; Transactivator
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| Gene Symbol |
Igs7
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| Gene Name |
intergenic site 7
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| Chromosome |
9
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| MGI Accession ID | show more close |
| Site of Expression |
TetO-inducible of EGFP and Cre-inducible widespread expression of tTA2 protein
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| Strain of Origin |
(129S6/SvEvTac x C57BL/6NCrl)F1
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| Molecular Note |
The reporter contains a Cre-dependent, tetracycline-activated tTA2-TRE transcriptional cassette combined with a Kozak consensus start site (for strong initiation of translation), two Myc-tags, an optimized G)22(-repeat MORF translation switch, and a farnesylated EGFP fluorescent reporter. The farnesylation sequence (CAAX domain from Ras) targets the reporter to membranous structures including neuronal dendrites and axons. Specifically, the final vector contains from 5' to 3': FRT2, 2x Ins, TRE promoter, loxP-flanked STOP sequence, a Kozak consensus start site (for strong initiation of translation),two Myc-tags, an optimized G)22(-repeat MORF switch, a farnesylated EGFP fluorescent reporter (EGFP-F), WPRE, pA, 2X Ins, CAG promoter, lox2272-flanked STOP sequence, tTA2, WPRE, pA and an attB-PGK-5';hygro-SD-frt5-SA 3';hygro-SV40pA-attPan FRT site, inserted into the T1 TIGRE locus.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
When maintaining a live colony, these mice are bred by homozygote sibling matings.
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| Breeding Strategy |
Homozygote x Homozygote
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For Research Use Only.
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