B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J

Tg(Thy1-Brainbow1.0)HLich

transgene insertion H

Reporter

Tg(Thy1-Brainbow1.0)HLich

transgene insertion H

UN

CFP, Cyan Fluorescent Protein, jellyfish; RFP, Red Fluorescent Protein, coral; YFP, Yellow Fluorescent Protein, jellyfish

3761285

tdimer2(12) (RFP), is expressed in peripheral and central neurons. When bred to Cre recombinase expressing mice, there is one of three expression outcomes for each transgene copy in each cell of the Cre expressing tissue(s): tdimer2(12) (RFP) (no recombination), mYFP, or mCerulean (CFP). Integration of 8 tandem transgene copies allows for labeling of individual neurons and glia with approximately 90 distinguishable color variations in Cre recombined cells.

C57BL/6 x CBA

The Thy1-Brainbow 1.0 transgene was designed with the mouse Thy1 regulatory elements surrounding three fluorescent protein (red (RFP), enhanced yellow (EYFP), or cyan (CFP)) sequences uniquely flanked with pairs of incompatible Lox sites. Specifically, this Brainbow 1.0 coding region contained (from 5'to 3') a Lox2272 site, LoxP site, tdimer2(12) RFP (with polyA sequence), Lox2272 site, monomeric EYFP (mYFPA206K; with membrane tethering palmitoylation sequence and polyA sequence), LoxP site, mCerulean CFP (with membrane tethering palmitoylation sequence and 5'FRT site and polyA sequence). This transgene was microinjected into C57BL/6JxCBA hybrid oocytes. The tdimer2(12) RFP is a non-oligomerizing DsRed variant with a 12 residue linker fusing two copies of the protein (tandem dimer). The monomeric EYFP (mYFPA206K) has an amino acid substitution replacing a hydrophobic region with a positively charged residue designed to prevent dimerization. The monomeric Cerulean (mCerulean) is a variant of ECFP (ECFPS72A/Y145A/H148D/A206K) with amino acid substitutions designed to improve spectral properties and prevent dimerization.

C57BL/6J

After many generations of breeding hemizygous mice together, only females have shown to be homozygous for the transgene; this suggests the transgene is X chromosome-linked. When maintaining a live colony, homozygous females may be bred to hemizygous males.

Homozygote x Hemizygote