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STOCK Gt(ROSA)26Sortm2(CAG-APX1*)Ddg/J
Cat. No.: CEMM-07250930
Common Name: ROSA26DR-ER-dAPEX2
ROSA26DR-ER-dAPEX2 mice conditionally express endoplasmic reticulum-targeted dimeric APEX2 (ER-dAPEX2, an enhanced soybean ascorbate peroxidase) in a cre and Flp dual recombinase-dependent manner. This multiplexed peroxidase-mediated electron microscopy (EM) labeling system facilitates the analysis of connectivity in the mammalian nervous system.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm2(CAG-APX1*)Ddg
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| Allele Name |
targeted mutation 2
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; No functional change
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| MGI Accession ID | show more close |
| Strain of Origin |
129S4/SvJae
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| Molecular Note |
A CAG promoter, FRT flanked Stop cassette, loxP-flanked Stop cassette (in reverse transcriptional orientation), ER-dAPEX2, WPRE, bGHpA, attB, PGK-Neo, PGKpA, and attP were introduced to intron 1 via homologous recombination. APEX2 is an enhanced (K14D, W41F, E112K, A134P) soybean ascorbate peroxidase (APX1) that is less catalytically active than horseradish peroxidase (HRP), but is functional when expressed in cytosolic environments, unlike HRP. Dimeric APEX2 (dAPEX2) contains the reversion of K14 and E112 and is presumed to enhance heme affinity and thermal stability of the protein. Targeting of dAPEX2 to the endoplasmic reticulum to Create ER-dAPEX2 was accomplished by a an N-terminal Igk signal sequence and C-terminal KDEL retention sequence. Flp-mediated recombination removed the FRT flanked stop cassette and left the floxed stop cassette intact.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
Wild-type from the colony
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| Breeding Considerations |
Heterozygotes and homozygotes are viable and fertile.
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| Breeding Strategy |
Heterozygote x Heterozygote
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For Research Use Only.
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