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B6;129-Nefltm1(cre/ERT)Nat/J

Cat. No.: CEMM-07250104

These targeted mutation mice carry a tamoxifen-inducible Cre cassette knocked into the 3' UTR of the Nefl, neurofilament, light polypeptide gene. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating 4-hydroxytamoxifen-induced, Cre-mediated targeted deletions specifically in large neurons.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Nefltm1(cre/ERT)Nat
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Allele Name
targeted mutation 1
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Allele Attributes
Recombinase-expressing; Inducible
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Gene Symbol
Nefl
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Gene Name
neurofilament
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Chromosome
14
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Expressed Genes
cre/ER, Cre inducible estrogen receptor, bacteriophage P1/Human
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MGI Accession ID
3777624
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Site of Expression
Projection neurons (after induction)
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Strain of Origin
129
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Molecular Note
An IRES-CreER cassette, a tetracycline resistance gene, and a Frt-flanked neomycin selection marker were all knocked in to the 3' UTR of the gene. The neomycin selection marker was removed by crossing to a C57BL/6 background Flp recombinase strain. Flp was then bred out of the line. When crossed with a Tg(CAG-Bgeo/ALPP)1Lbe reporter strain and given tamoxifen, Cre expression was detected in ganglion cells and horizontal cells of the retina, and in a sparse number of neurons in the brain.
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HUSBANDRY
Suggested Controls
B6129SF1/J B6129SF2/J
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Breeding Considerations
When maintained as a live colony, homozygous mice may be bred. Although the donating investigator reports that homozygous males may not be fertile and homozygous females may have reduced fertility, homozygous colonies have no reported fertility problems.
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For Research Use Only.
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