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B6;129S-Gt(ROSA)26Sortm35.1(CAG-aop3/GFP)Hze/J
Cat. No.: CEMM-07250244
Common Name: Ai35D; Ai35(RCL-Arch/GFP)
The Ai35D (or Ai35Δneo) allele has a floxed-STOP cassette preventing transcription of the downstream Arch-GFP fusion gene. Exposure to Cre recombinase that deletes the STOP cassette results in Arch-GFP expression. These Ai35D mice are useful for optogenetic studies to express an inhibitory opsin that effectively silences the activity of cortical neurons (and perhaps other excitable cell types such as muscle cells and immune cells).
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm35.1(CAG-aop3/GFP)Hze
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| Allele Name |
targeted mutation 35.1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
AOP3, Archaerhodopsin, Halorubrum sodomense; GFP, Green Fluorescent Protein
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| MGI Accession ID | show more close |
| Site of Expression |
Cre excision of the stop signal results in expression of an Archaerhodopsin-3 protein/EGFP fusion protein (Arch-GFP) in Cre-expressing tissues.
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| Strain of Origin |
(129S6/SvEvTac x C57BL/6NCrl)F1
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| Molecular Note |
The Rosa-CAG-LSL-Arch-GFP-WPRE targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), an Arch-GFP fusion gene, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a BGH polyA signal, and an attB/attP-flanked PGK-FRT Neo-polyA cassette. The Arch-GFP fusion gene is flanked with small domain modifications designed to facilitate correct processing and localization of newly synthesized Arch-GFP protein. Specifically, this ss-Arch-GFP-ER2 construct was designed with the signal sequence (ss) from truncated MHC class I antigen (amino acids MVPCTLLLLLAAALAPTQTRA), a mammalian codon-optimized cDNA sequence encoding the Archaerhodopsin-3 protein (Arch, aR-3, or aop3 ; derived from the halophilic bacterium Halorubrum sodomense) fused in-frame via a 15 nucleotide linker to the amino terminus of a green fluorescent protein (GFP), and an endoplasmic reticulum exporting sequence (ER2) from the potassium channel Kir2.1 gene (amino acids FCYENEV) at the C-terminal of the GFP. PhiC31-mediated recombination replaced the neo cassette with the recombined attB/attP site (attL).
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
Homozygous mice are viable and fertile. When maintaining a live colony, homozygous mice may be bred together.
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| Breeding Strategy |
Homozygote x Homozygote
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For Research Use Only.
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