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STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
Cat. No.: CEMM-07250022
Common Name: R26SmoM2
When these mice are used in conjunction with a Cre recombinase-expressing strain, successful Cre-mediated excision results in the constitutive expression of the Smo/EYFP fusion gene (monitored by using EYFP-specific fluorescence protocols) and unrestrained Hedgehog signaling in Cre-expressing tissues. As examples, this strain may be useful in studies of tumorigenic potential in the hedgehog pathway, progenitor cell involvement in the development of medulloblastomas, craniofacial development, thalamic development, and of oligodendrocyte maturation and CNS myelination when used in combination with specific cre-expressing strains.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm1(Smo/EYFP)Amc
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Constitutively active; Inserted expressed sequence
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
YFP, Yellow Fluorescent Protein, jellyfish
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| MGI Accession ID | show more close |
| Site of Expression |
When crossed to a Cre recombinase-expressing strain, expression of Enhanced Yellow Fluorescent Protein and the mouse smoothened homolog (Drosophila) protein is observed in the Cre-expressing tissues, leading to unrestrained Hedgehog signaling
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| Strain of Origin |
129X1/SvJ
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| Molecular Note |
A loxP flanked PGK-neo-stop cassette upstream of the Yellow Fluorescent Protein (YFP) and mouse gene (SMO/EYFP) was inserted into the locus. The mutant allele consists of a fusion product involving YFP and the constitutively active W539L point mutation of the mouse gene (SmoM2), which corresponds to the W535L mutation in the human sequence. Expression of the fusion gene is blocked by a loxP-flanked STOP fragment placed between the endogenous promoter and the fusion sequence.
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| HUSBANDRY | |
|---|---|
| Breeding Strategy |
Homozygote x Homozygote
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For Research Use Only.
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