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B6.Cg-Mapkapk2tm1.1Yaff/J

Cat. No.: CEMM-07250534

Common Name: MK2CV
MK2CV has exon 2 of the MAP kinase-activated protein kinase 2 gene flanked with loxP sites in opposing orientations. A single exposure to Cre recombinase inverts exon 2 - this generates a STOP codon and results in a functional null allele. In addition, exon 2 can switch reversibly between the MK2-expressing and MK2-null orientation as long as Cre recombinase is active in the cell. These MK2CV mice may be useful in studying the p38 MAP kinase/MK2 pathway, other checkpoint control pathways, DNA damage response, DNA repair, cell cycle arrest and apoptosis - as well as with other cancer models to study sensitization of tumors to DNA-damaging chemotherapeutics in vivo.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Mapkapk2tm1.1Yaff
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Allele Name
targeted mutation 1.1
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Allele Attributes
Conditional ready (e.g. floxed); No functional change
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Gene Symbol
Mapkapk2
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Gene Name
MAP kinase-activated protein kinase 2
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Chromosome
1
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MGI Accession ID
5528531
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Strain of Origin
(C57BL/6 x 129S4/SvJae)F1
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Molecular Note
A targeting vector was designed to insert a loxP site upstream of exon 2, and a second loxP site (in reverse complement sequence to the first loxP site) downstream of exon 2 as well as a frt:PGK-neo:FRT sequence downstream of the second loxP site. Because inward-facing loxP sequences result in Cre-mediated inversion rather than excision, exon 2 can reversibly switch from Mapkap2 expression orientation (MK2, CV; Cre-versible) to the null orientation (MK2) and back as long as Cre recombinase is active in the cell.FLP recombinase mediated recombination removed the neomycin cassette.
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HUSBANDRY
Suggested Controls
C57BL/6J
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Breeding Considerations
When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice. Alternatively, homozygous mice may be bred together.
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For Research Use Only.
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