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STOCK Gt(ROSA)26Sortm1(CAG-Syp/EYFP)Htng/J
Cat. No.: CEMM-07250918
Common Name: FSF-LSL-SypYFP; R26-CAG-FSF-LSL-SypYFP
FSF-LSL-SypYFP (or R26-CAG-FSF-LSL-SypYFP) is a dual-recombinase intersectional allele that has a frt-flanked STOP and loxP-flanked STOP that prevent transcription of a synaptophysin-YFP fusion protein. Exposure to FLP recombinase and Cre recombinase results in YFP expression enriched at the synapse/synaptic vesicle (axonal boutons [terminal and en passant]) of the neuron subtype(s) defined by the overlap of FLP/Cre expression.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm1(CAG-Syp/EYFP)Htng
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| MGI Accession ID | show more close |
| Strain of Origin |
(C57BL/6 x 129S4/SvJae)F1
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| Molecular Note |
The targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), an FRT flanked STOP cassette (with a short open reading frame, followed by translational stops in all three reading frames, a synthetic polyA sequence, a HGH polyA sequence and a HSV-TK polyA sequence), a loxP-flanked STOP cassette (with stop codons in all three reading frames linked to three repeats of the SV40 polyA signal), the SypYFP fusion gene (a mouse synaptophysin [Syp] cDNA fused to an enhanced yellow fluorescent protein sequence), a woodchuck hepatitis virus post-transcriptional regulatory element, a BGH polyA signal, an AttB site, a PGK promoter-Neomycin resistance gene-PGK pA cassette and an AttP site. This entire targeting vector was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
Wild-type from the colony
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| Breeding Considerations |
Mice homozygous for the R26-CAG-FSF-LSL-SypYFP (or FSF-LSL-SypYFP) allele are viable and fertile with normal breeding, and no gross physical or behavioral abnormalities.When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred animals. Alternatively, homozygous mice may be bred together.
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For Research Use Only.
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