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B6;129-Gt(ROSA)26Sortm2Nat/J
Cat. No.: CEMM-07250158
Common Name: ROSA26-AP Inversion (R26iAP)
This strain carries an inverted human placental alkaline phosphatase (ALPP; PLAP) coding segment flanked by oppositely-oriented loxP sites integrated in the Gt(ROSA)26Sor gene. Cre excision of the floxed segment flips rather than excises the ALPP inversion and reinstates a transcriptionally active form of the gene. This reporter can be activated by tissue-specific cre recombinase in any cell in the body at any time in development. The result is very clear ALPP enzyme activity that can be detected histochemically.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm2Nat
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| Allele Name |
targeted mutation 2
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| Allele Attributes |
Reporter
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
ALPP, alkaline phosphatase, placental, human
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| MGI Accession ID | show more close |
| Strain of Origin |
129
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| Molecular Note |
The human placental alkaline phosphatase (ALPP; PLAP) coding region was engineered to contain a single intron with a loxP site. Another loxP site in inverted orientation was placed 3' of the ALPP coding region. A single round of Cre-mediated recombination in E. coli resulted in the inversion of the 3' half of the coding region, thereby disrupting the ALPP open reading frame. This construct, referred to as "AP inversion" or "AP inv", was knocked into the ubiquitously-expressed Gt(ROSA)26Sor locus by homologous recombination.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
B6129SF2/J
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| Breeding Considerations |
When maintained as a live colony, homozygotes or heterozygotes may be bred together.
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For Research Use Only.
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