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STOCK Gt(ROSA)26Sortm1(CAG-EYFP*, -mEYFP*, -tdTomato*, -mTFP1*)Ben/J

Cat. No.: CEMM-07250854

Common Name: iMb2-Control-Mosaic
iMb2-Control-Mosaic mice allow three possible Cre recombination outcomes in any single cell. Each outcome results in expression of a membrane-bound fluorescent protein, and each is mutually exclusive and permanent. These mice are also designed to permit further introduction of genes of interest for inducible, fluorescent and functional genetic mosaic analysis (ifgMosaic). Mice are available with the similarly-designed iChr2-Control-Mosaic allele harboring chromatin/nuclear-targeted fluorescent proteins. In addition, the iMb2-Control-Mosaic::iChr2-Control-Mosaic compound heterozygous mice harbor both alleles.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Gt(ROSA)26Sortm1(CAG-EYFP*,-mEYFP*,-tdTomato*,-mTFP1*)Ben
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Allele Name
targeted mutation 1
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Allele Attributes
Conditional ready (e.g. floxed); Reporter
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Gene Symbol
Gt(ROSA)26Sor
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Gene Name
gene trap ROSA 26
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Chromosome
6
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Expressed Genes
RFP, Red Fluorescent Protein, coral; CFP, Cyan Fluorescent Protein, jellyfish; GFP, Green Fluorescent Protein; HA tag, HA tag; YFP, Yellow Fluorescent Protein, jellyfish
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MGI Accession ID
6108157
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Site of Expression
Fluorescent proteins will be expressed in in tissues expressing Cre recombinase driven by a selected promoter.
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Strain of Origin
(129S6/SvEvTac x C57BL/6NCrl)F1
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Molecular Note
This allele has three membrane-targeted fluorescent protein sequences - each uniquely flanked with pairs of incompatible lox sites (in the same direction) inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus with a CAG hybrid promoter/enhancer. The targeting vector is designed with (from 5' to 3') two copies of chicken beta-globin HS4 insulator element (2xINS; inCrease downstream gene expression and minimize regulatory interference), a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a loxN site, a lox2272 site, a loxP site, the non-cytotoxic/non-fluorescent Phialidium yellow fluorescent protein variant PhiYFP with nuclear localization signal (N-PhiMut), SV40 polyA signal sequence, a second loxN site, a full consensus KOZAK sequence, the Mb2-YFP gene (monomeric enhanced yellow fluorescent protein [mYFPA206K] with N-terminal 41 amino acid membrane-targeting tag sequence), in-frame Thosea asigna virus 2A cleavage site (T2A; mediates ribosomal skipping), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance mRNA transcript stability), SV40 polyA signal sequence, a second lox2272 site, a full consensus KOZAK sequence, the Mb2-Tomato gene (non-oligomerizing tandem-dimer DsRed fluorescent protein variant tdTomato with N-terminal 41 amino acid membrane-targeting tag sequence), in-frame T2A sequence, WPRE sequence, SV40 polyA signal sequence, a second loxP site, a full consensus KOZAK sequence, the Mb2-HA-TFP1 gene (monomeric teal fluorescent protein mTFP1 with N-terminal hemagglutinin tag and 41 amino acid membrane-targeting tag sequence), in-frame T2A sequence, WPRE sequence, SV40 polyA signal sequence, an FRT site flanked neomycin resistance gene cassette, and a second 2xINS sequence.
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HUSBANDRY
Suggested Controls
B6129SF2/J
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Breeding Considerations
Before exposure to Cre recombianse, the donating investigator reports that homozygous mice are viable and fertile with normal breeding, and exhibit no behavioral, neurological or any other spontaneous abnormalities. When maintaining a live colony, homozygous mice may be bred together.
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Breeding Strategy
Homozygote x Homozygote
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For Research Use Only.
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