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B6;129S-Gt(ROSA)26Sortm65.1(CAG-tdTomato)Hze/J
Cat. No.: CEMM-07250461
Ai65(RCFL-tdT)Δneo mice (also called Ai65(RCFL-tdT)-D, Ai65Δneo, or Ai65D) harbor the Rosa-CAG-FrtSTOPFrt-LoxSTOPLox-tdTomato::deltaNeo conditional allele designed with both an frt-flanked STOP cassette and a loxP-flanked STOP cassette preventing transcription of the tdTomato fluorescent protein. After removal of the flanked STOP cassettes by FLP- and Cre-recombination, tdTomato fluorescence is expected in cells/tissues expressing both FLP recombinase and Cre recombinase.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm65.1(CAG-tdTomato)Hze
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| Allele Name |
targeted mutation 65.1
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| Allele Attributes |
Conditional ready (e.g. floxed); No functional change
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| MGI Accession ID | show more close |
| Site of Expression |
Expression of tdTomato fluorescence depends upon promoter used that drives both FLP and Cre recombinase.
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| Strain of Origin |
(129S6/SvEvTac x C57BL/6NCrl)F1
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| Molecular Note |
The Rosa-pCAG-FSF-LSL-tdTomato-WPRE-bGHpA-AttB-PGK-Neo-polyA-AttP targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), an FRT flanked STOP cassette (with a short open reading frame, followed by translational stops in all three reading frames, a synthetic polyA sequence, a polyA sequence from the human growth hormone gene, and a polyA sequence from the Herpes Simplex Virus TK gene), a loxP-flanked STOP cassette (with stop codons in all three reading frames linked to three repeats of the SV40 polyA signal), a sequence encoding the tdTomato fluorescent protein (a non-oligomerizing DsRed fluorescent protein variant with a 12 residue linker fusing two copies of the protein (tandem dimer)), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a bovine growth hormone polyA signal, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, and an AttP site. This entire targeting vector was inserted between exons 1 and 2. PhiC31-mediated recombination removed the AttB/AttP-flanked PGK-Neo-polyA cassette and replace it with the recombined AttB/AttP site (AttL).
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For Research Use Only.
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