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STOCK Gt(ROSA)26Sortm1(CAG-Clomeleon*)Kjst/J
Cat. No.: CEMM-07251114
Common Name: SuperClomeleon; sClm Lox-P
sCLM-floxed KI mice allow Cre-inducible expression of SuperClomeleon, an improved fluorescent fusion protein containing Cerulean and YFP (topaz) that acts as a ratiometric indicator for chloride ions. This strain may be useful for detecting inhibitory synaptic activity in single mouse neurons.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm1(CAG-Clomeleon*)Kjst
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| MGI Accession ID | show more close |
| Strain of Origin |
129/Sv
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| Molecular Note |
The targeting vector is designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAAG), a loxP-flanked STOP/Neo cassette, SuperClomeleon (an improved fluorescent fusion protein containing Cerulean and YFP) and a polyA sequence. SuperClomeleon differs from Clomeleon by the following additions: a short interdomain linker, an S30R point mutation in both domains, intermediate affinity-tuning mutations, Q69T/V163A double mutant in YFP and replacement of the CFP domain with Cerulean. It provides a higher choloride sensitivity than Clomeleon.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
129S1/SvImJ
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| Breeding Considerations |
Mice homozygous for the mutation are viable and fertile.
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| Breeding Strategy |
Heterozygote x Heterozygote
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For Research Use Only.
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