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STOCK Grm7Tg(SMN2)89Ahmb Smn1tm3(SMN2/Smn1)Mrph Tg(CAG-cre/Esr1*)5Amc Tg(SMN2*delta7)4299Ahmb/J
Cat. No.: CEMM-07250130
Common Name: SMN2; Δ7; Cre-ER; SmnRes; SMN2; Δ7; Cre-ER; SmnCOIN; Delta 7 mouse with hybrid rescue allele and tamoxifen inducible Cre
The severely affected "rescuable" SMA model mice, SMN2;Δ7;Cre-ER;SmnRes/Res, allow tamoxifen-inducible rescue of Type II (moderate) proximal spinal muscular atrophy.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Grm7Tg(SMN2)89Ahmb
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| Allele Name |
transgene insertion 89
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| Allele Attributes |
Hypomorph; Inserted expressed sequence; Humanized sequence
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| Gene Symbol |
Grm7
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| Gene Name |
glutamate receptor
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| Chromosome |
6
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| Expressed Genes |
SMN2, survival of motor neuron 2, centromeric, human
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| MGI Accession ID | show more close |
| Site of Expression |
Human SMN2 protein is expressed in the tissues examined (brain, liver, spinal cord).
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| Strain of Origin |
FVB/N
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| Molecular Note |
A 35.5 kb genomic fragment containing the human survival motor neuron 2 (SMN2) gene and promoter was used for the transgene. The transgene is ubiquitously expressed in all tissues examined by Northern blot analysis. Line 89 carries 1 copy of the transgene integrated into intron 4 of the gene. RT-PCR confirmed reduced expression of the gene the transgene is integrated into.
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| HUSBANDRY | |
|---|---|
| Breeding Considerations |
Mice heterozygous for the Smn1tm3(SMN2/Smn1)Mrph mutation (SmnRes/+) and homozygous for the other mutations/transgenes are viable and fertile with no reported abnormalities or symptoms of neuropathology. As such, our repository is maintained as heterozygous for the Smn1tm3(SMN2/Smn1)Mrph mutation (SmnRes/+), homozygous for the Tg(SMNΔ7)4299 transgene (SMNΔ7+/+), homozygous for the Tg(SMN2)89 transgene (SMN2+/+), and hemizygous for the Tg(CAG-CreER)5 transgene (Cre-ER). That is, SmnRes/+;SMNΔ7+/+;SMN2+/+;Cre-ER+/-. A researcher must breed SmnRes/+;SMNΔ7+/+;SMN2+/+;Cre-ER+/- mice together to generate the severely affected "rescuable" SMA model (SMN2;Δ7;Cre-ER;SmnRes/Res).
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For Research Use Only.
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