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B6(Cg)-Slc17a8em1(cre/ERT2)Zyliu/J
Cat. No.: CEMM-07250994
Common Name: vGlut3-P2A-iCreER
vGlut3-P2A-iCreERT2 mice carry a CRISPR/Cas9 generated insertion of a P2A-iCre/ERT2 cassette in exon 12, immediately before the stop codon of the Slc17a8 gene, without disrupting endogenous vesicular glutamate transporter 3 expression. These mice may be used to generate tamoxifen-induced conditional mutations for studying gain-or-loss of function and/or fate mapping related to glutamatergic synaptic vesicle trafficking.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Slc17a8em1(cre/ERT2)Zyliu
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| Allele Name |
endonuclease-mediated mutation 1
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| Allele Attributes |
Recombinase-expressing; Inducible
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| Gene Symbol |
Slc17a8
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| Gene Name |
solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter)
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| Chromosome |
10
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| MGI Accession ID | show more close |
| Site of Expression |
Inner hair cells (IHCs).
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| Strain of Origin |
C57BL/6N
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| Molecular Note |
A plasmid encoding a guide RNA and PAM sequence TGG was designed to introduce a P2A peptide fused to an Cre/ERT2 cassette into exon 12, immediately before the stop codon, of the solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 8 (Slc17a8) gene. Guide RNA and the Cas9 nuclease were introduced into the cytoplasm of C57BL/6N-derived fertilized eggs with well recognized pronuclei.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6NJ
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| Breeding Considerations |
When maintaining a live colony, homozygous mice may be bred together.
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| Breeding Strategy |
Heterozygote x Heterozygote
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For Research Use Only.
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