STOCK Et(cre)TFC.09Lois/J

Cat. No.: CEMM-07250550

Common Name: Thy1.2-Cre transgenic line TFC.09

The Thy1.2-Cre enhancer trap transgenic mouse line 09 (TFC.09) expresses Cre recombinase sparsely in a small number of cortical progenitor cells during early forebrain development. GFP expression is almost undetectable by fluorescence and it is only transiently expressed in a few stem cells in the embryo. TFC.09 mice allow examination of individual columns and cells, and may be useful for investigating patterns of neural development in the neocortex, as well as for studying cell lineage in mouse brain.

Inquiry

Status	Live Mouse Frozen Embryo
Age	4 weeks 12 weeks Customized Age
Sex	Male Female

GENETICS
Allele Symbol	Et(cre)TFC.09Lois show more close
Allele Name	enhancer trap TFC.09 show more close
Allele Attributes	Recombinase-expressing show more close
Gene Symbol	Et(cre)TFC.09Lois show more close
Gene Name	enhancer trap TFC.09 show more close
Chromosome	X show more close
Expressed Genes	Cre, Cre recombinase, bacteriophage P1 show more close
MGI Accession ID	5443915 show more close
Site of Expression	Cre recombinase is sparsely expressed in a small number of cortical progenitor cells during early forebrain development. show more close
Strain of Origin	C57BL/6J show more close
Molecular Note	Injection of a lentiviral vector carrying an enhancer trap detector cassette composed of Cre::GFP fusion gene under control of the mouse Thy1.2 minimal promoter (129 bp) was used to generate several Cre lines. These were crossed with Z/EG reporter mice, and the patterns of GFP labeling in the brains of the offspring were examined. One line, TCF.09, displayed a low rate of recombination in a sparse and seemingly random subset of neural precursors during development. A single copy of the transgene integrated into the seventh intron of the glypican-3 gene; recombination activity showed no correlation with endogenous Gpc3 gene expression. The investigator reports that GFP expression is almost undetectable by fluorescence and it is only transiently expressed in a few stem cells in the embryo. show more close

HUSBANDRY
Breeding Considerations	The Thy1.2-Cre enhancer trap transgene integrated as a single copy into the seventh intron of the glypican 3 gene on the X chromosome. When maintaining a live colony, hemizygous mice may be bred together, to wildtype (non-carrier) siblings, or to C57BL/6J inbred mice. The donating investigator breeds homozygous females with hemizygous males, and reports those animals are viable and fertile with normal lifespan and no reported abnormalities in behavior or brain structure. show more close
Coat Color	Agouti, black and albino coat colors are observed in this colony. show more close

HUSBANDRY

Breeding Considerations

The Thy1.2-Cre enhancer trap transgene integrated as a single copy into the seventh intron of the glypican 3 gene on the X chromosome. When maintaining a live colony, hemizygous mice may be bred together, to wildtype (non-carrier) siblings, or to C57BL/6J inbred mice. The donating investigator breeds homozygous females with hemizygous males, and reports those animals are viable and fertile with normal lifespan and no reported abnormalities in behavior or brain structure.