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B6.Cg-Tg(CAG-Bgeo, -DsRed*MST)1Nagy/J

Cat. No.: CEMM-07250034

These Z/RED transgenic mice express beta-galactosidase (lacZ) under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with red fluorescent protein (DsRed*MST) expression in tissues expressing Cre recombinase. This double reporter system makes it possible to distinguish a lack of reporter expression from a lack of Cre recombinase expression while providing a means to assess Cre excision activity in live animals and cells. This strain is discontinued, please see the replacement strain:.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Tg(CAG-Bgeo,-DsRed*MST)1Nagy
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Allele Name
transgene insertion 1
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Allele Attributes
Reporter
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Gene Symbol
Tg(CAG-Bgeo,-DsRed*MST)1Nagy
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Gene Name
transgene insertion 1
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Chromosome
UN
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Expressed Genes
Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes, E. coli; RFP, Red Fluorescent Protein, coral
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MGI Accession ID
3697095
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Site of Expression
LacZ is expressed in embryonic and adult tissues; When crossed with a Cre recombinase-expressing strain, LacZ expression is replaced with Red Fluorescent Protein variant (DsRed.MST) expression
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Strain of Origin
(129S6/SvEvTac x C57BL/6NCrl)F1
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Molecular Note
The transgene contains the coding sequence for the DsRed*MST variant red fluorescent protein and a polyadenylation signal downstream of a loxP-flanked lacZ/neomycin resistance fusion gene (Betageo) followed by three polyadenylation signals (STOP sequence); both coding sequences reside downstream of a chicken beta-actin promoter and a cytomegalovirus enhancer. Excision of the lacZ-STOP by Cre recombinase in doubly transgenic ES cells or mice leads to expression of DsRed*MST; use of a ubiquitously expressed Cre transgene results in fluorescence of ES cells, embryos and adult mice.
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HUSBANDRY
Suggested Controls
C57BL/6J Noncarrier
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Breeding Considerations
It has been our experience that hemizygotes maintained on a mixed genetic background are often smaller than littermates and subject to postnatal mortality. Delayed weaning greatly enhances the survival. Additionally, smaller mice or mice with improper tooth development may benefit from adding pulverized (and/or increased fat) chow to the cage floor prior to and after weaning to promote the survival of the transgenic pups. Although homozygous animals are born, animals have not survived past 5 weeks of age. Given this, as well as the possibility that fluorescent protein polymer formation may result in DsRed-expressing mice, hemizygous mice are bred to wildtype siblings or to C57BL/6J mice.
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Breeding Strategy
Inbred x Hemizygote
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For Research Use Only.
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