B6.Cg-Nxph4^{tm1.1(cre/ERT2)Hze}/J

Nxph4^{tm1.1(cre/ERT2)Hze}

targeted mutation 1.1

Recombinase-expressing; Inducible

Nxph4

neurexophilin 4

10

cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene

5501233

Following Tamoxifen induction, reporter gene expression is found in cortical layer 6b neurons and in other scattered small cells (possibly glia) throughout the cortex.

(129S6/SvEvTac x C57BL/6NCrl)F1

A targeting vector contained a partial Nxph4 intron sequence containing an frt3 site, a partial Nxph4 exon 2 sequence up to (but not including) the endogenous stop codon, a self-cleavingT2A sequence in-frame with the Nxph4 coding sequence, a Cre/ERT2 fusion gene, a bovine growth hormone polyA signal, an AttB site, a PGK-Neo-polyA cassette, an frt5 site, an mRNA splice acceptor, the 3' portion of the hygromycin gene with SV40 polyA signal, and an AttP site. This targeting vector was electroporated into G4 ES cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were bred to PhiC31-expressing mice to delete the AttB/AttP-flanked sequences and replace it with the recombined AttB/AttP site (AttL).

C57BL/6J Wild-type from the colony

When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or to C57BL/6J inbred mice. The phenotype of homozygous mice has not yet been determined.