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B6(Cg)-Ssttm1(cre/ERT2)Zjh/J

Cat. No.: CEMM-07250199

Common Name: Sst-CreERT2 knock-in/knock-out
The Sst-CreERT2 knock-in/knock-out allele in these mice both abolishes Sst (somatostatin) gene function and expresses a CreERT2 fusion protein from the Sst promoter/enhancer elements.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Ssttm1(cre/ERT2)Zjh
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Allele Name
targeted mutation 1
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Allele Attributes
Recombinase-expressing; Inducible
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Gene Symbol
Sst
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Gene Name
somatostatin
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Chromosome
16
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Expressed Genes
cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene
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MGI Accession ID
4355153
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Site of Expression
Tamoxifen-inducible Cre recombinase activity is observed in somatostatin-positive neurons.
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Strain of Origin
C57BL/6
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Molecular Note
A targeting vector was designed to insert a CreERT2 fusion gene (Cre-ERT2; Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), an SV40 polyA signal, and an FRT flanked neo cassette into the initiation codon of the Sst (somatostatin) gene. This construct was electroporated into C57BL/6 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with white C57BL/6 mice to originate the colony. Mutant mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background (N10)) to remove the neo selection cassette. These Sst-CreERT2 mice were subsequently bred together (selectively removing the FLPe transgene).
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HUSBANDRY
Suggested Controls
C57BL/6J Wild-type from the colony
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Breeding Considerations
When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings, or to C57BL/6J mice.
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For Research Use Only.
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