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STOCK En2tm6Alj/J
Cat. No.: CEMM-07250138
These En2lox mice have loxP sites flanking the engrailed homeodomain portion in exon 2 of the engrailed 2 gene. These mice may be used to generate conditional mutations for studying engrailed protein function in the developing mesencephalon, rhombomere 1, and jaw muscles, as well as the embryonic and adult cerebellum.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
En2tm6Alj
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| Allele Name |
targeted mutation 6
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| Allele Attributes |
Conditional ready (e.g. floxed); No functional change
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| Gene Symbol |
En2
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| Gene Name |
engrailed 2
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| Chromosome |
5
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| MGI Accession ID | show more close |
| Strain of Origin |
129S6/SvEvTac
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| Molecular Note |
The targeting construct contains a 3.1 kb En2 5' fragment, an Frt-flanked PGK-neo cassette followed by a LoxP site in the En2 intron and a 4.8 kb En2 genomic fragment containing a LoxP site in the XbaI site of the 3' UTR. Flpe-mediated recombination deleted the PGK-neo, producing an En2 allele with a floxed region.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
Wild-type from the colony
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| Breeding Considerations |
When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or with C57BL/6J inbred mice. As the donating investigator reports that homozygous mice are viable and fertile, the colony may also be maintained by breeding homozygous mice together.
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For Research Use Only.
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