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B6.Cg-Rln3tm1.1(cre/GFP)Et/J

Cat. No.: CEMM-07250967

Common Name: Rln3-Cre; Rln3-IRES-Cre/GFP
Rln3-Cre mice (also called Rln3-IRES-Cre/GFP) are designed to have the endogenous relaxin 3 promoter/enhancer driving Cre/GFP fusion protein expression to nucleus incertus (NI) of the pontine tegmentum (a subset of NI GABAergic neurons) and to smaller populations of Rln3-expressing neurons found in the periaqueductal gray and adjacent to the substantia nigra. While Rln3-Cre was designed to retain endogenous Rln3 expression, this allele is hypomorphic; as such it is recommended that Rln3-Cre heterozygotes be used for Cre-lox applications. These mice may be used to generate conditional mutations in RLN3+ cells for studying physiological processes such as stress, memory and appetite regulation, as well as neuropeptide signaling processes.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Rln3tm1.1(cre/GFP)Et
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Allele Name
targeted mutation 1.1
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Allele Attributes
Recombinase-expressing; Reporter
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Gene Symbol
Rln3
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Gene Name
relaxin 3
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Chromosome
8
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MGI Accession ID
6356529
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Site of Expression
Endogenous relaxin 3 promoter/enhancer directs Cre/GFP fusion protein expression to nucleus incertus (NI) of the pontine tegmentum (a subset of NI GABAergic neurons) and to smaller populations of Rln3-expressing neurons found in the periaqueductal gray and adjacent to the substantia nigra.
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Strain of Origin
(129S6/SvEvTac x C57BL/6NCrl)F1
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Molecular Note
The targeting vector contained, from 5' to 3', an internal ribosome entry site sequence (IRES), a Cre/GFP fusion protein sequence and a FRT flanked neo cassette inserted into the 3'UTR in exon 2 of the relaxin 3 gene (Rln3) on chromosome 8. Chimeric mice were bred to FLPe mice for removal of the neo cassette.
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HUSBANDRY
Suggested Controls
C57BL/6NJ
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Breeding Considerations
Mice heterozygous or homozygous for this Rln3-Cre allele are viable and fertile with normal breeding. While Rln3-Cre was designed to retain endogenous Rln3 expression, the donating investigator reports that for unknown reasons this allele has hypomorphic expression of RLN3 (antibody staining suggests that it has less than half the wildtype allele expression). As such, they use either heterozygous or homozygous mice for breeding, but do not use homozygous mice for any Cre-lox studies. When maintaining our live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6NJ inbred mice. Alternatively, homozygous mice may be bred together for colony maintenance.
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Breeding Strategy
Wild-type x Heterozygote; Heterozygote x Wild-type
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For Research Use Only.
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