C57BL/6N-Tg(Npy-EGFP/RNAi:Gad1)1Mirn/J

Cat. No.: CEMM-07250680

Common Name: Tg(Npy-eGFP/miRNA:Gad1)1KM; NPY1; NPYGAD1TG; Npy/Gad1

The Npy-eGFP-miGAD67 line 1 BAC transgenic mouse line is a hybrid reporter/miRNA knockdown model where neuropeptide Y interneuronal expression of a synthetic Gad1 miRNA precursor results in Gad1 transcript downregulation (silencing of GAD1 expression) via endogenous miRNA processing mechanisms. These mice may be useful in studying NPY+ GABAergic interneurons in behavioral and molecular processes, as well as cell-type specific GABAergic disturbances in manifestations of schizophrenia.

Inquiry

Status	Live Mouse Frozen Embryo
Age	4 weeks 12 weeks Customized Age
Sex	Male Female

GENETICS
Allele Symbol	Tg(Npy-EGFP/RNAi:Gad1)1Mirn show more close
Allele Name	transgene insertion 1 show more close
Allele Attributes	Conditional ready (e.g. floxed); Reporter; Knockdown show more close
Gene Symbol	Tg(Npy-EGFP/RNAi:Gad1)1Mirn show more close
Gene Name	transgene insertion 1 show more close
Chromosome	UN show more close
Expressed Genes	GFP, Green Fluorescent Protein; Gad1, glutamate decarboxylase 1, mouse, laboratory show more close
MGI Accession ID	5703795 show more close
Site of Expression	The transgene is specifically expressed in the cell sub-populations that normally express the Npy gene; including interneuron neurogliaform cells and Martinotti cells. show more close
Strain of Origin	C57BL/6NHsd show more close
Molecular Note	The transgenic construct uses 160 kbp from bacterial artificial chromosome (BAC) RP24-386I9 containing the neuropeptide Y locus (Npy) as well as ~72 kbp of centromeric sequences and ~81 kbp of telomeric sequences; no other confirmed genes are present in the flanking sequences. Using homologous recombination/BAC recombineering, the following is inserted in-frame with the start codon of the BAC-encoded Npy: eGFP, a lox2272 site, a human -globin minigene (exon1-intron-exon2) with the intron containing a miRNA:Gad1 sequence, a second lox2272 site and an SV40 polyA signal. An FRT flanked neomycin resistance cassette was also present, but removed during BAC recombineering in vitro. The miRNA:Gad1 sequence is a fused miR30, miRNA:Gad1 synthetic miRNA sequence directed against the mouse glutamic acid decarboxylase 1 (Gad1 or GAD67) mRNA (mp ID 283874), with unique flanking miR-30 sequences. No other loci on the BAC were altered. show more close

HUSBANDRY
Suggested Controls	C57BL/6NJ show more close
Breeding Considerations	The donating investigator reports that hemizygous and homozygous mice are viable and fertile. When maintaining a live colony, hemizygous mice may be bred together, to wildtype (non-carrier) mice from the colony, or to C57BL/6NJ inbred mice. Alternatively, homozygous mice may be bred together. show more close

For Research Use Only.