STOCK Cck^{tm2.1(cre/ERT2)Zjh}/J

Cck^{tm2.1(cre/ERT2)Zjh}

targeted mutation 2.1

Recombinase-expressing; Inducible

Cck

cholecystokinin

9

cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene

5014094

Following Tamoxifen administration, Cre recombinase activity is observed in cholecystokinin positive neurons in cortex (both interneurons and pyramidal neurons).

C57BL/6

A targeting vector was designed to insert a CreERT2 fusion gene (Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), an SV40 polyA signal, and an FRT flanked neo cassette into the initiation codon of the cholecystokinin locus (Cck). This construct was electroporated into C57BL/6-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with C57BL/6 mice to originate the colony. Mutant mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background (N10)) to remove the neo selection cassette. These Cck-CreER (or Cck-CreERT2) mice were subsequently bred to C57BL/6 inbred mice for two additional generations and then bred together for many more generations (and the FLPe transgene was removed).

Wild-type from the colony

When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings, or to C57BL/6J mice. The donating investigator reports that homozygous mice are viable and fertile.