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B6.Cg-Gt(ROSA)26Sortm1(CAG-Eos*/Calm*)Mrc/J
Cat. No.: CEMM-07250982
Common Name: R26-CAG-lsl-CaMPARI2; CaMPARIlsl
R26-CAG-lsl-CaMPARI2 mice express CaMPARI2 fluorescent calcium reporter from a CAG promoter introduced to the Gt(ROSA)26Sor gene when a loxP-flanked STOP cassette is excised with Cre recombinase. CaMPARI changes from green to red fluorescence with high intracellular calcium and photoconversion light, and is thus useful in monitoring neuronal activity. A poster describing the strain can be found here.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm1(CAG-Eos*/Calm*)Mrc
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| MGI Accession ID | show more close |
| Strain of Origin |
(129S6/SvEvTac x C57BL/6NCrl)F1
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| Molecular Note |
The targeting construct contains a CAG promoter followed by a loxP-Stop-loxP-CaMPARI2::attB-PGK-FRT Neo-pA-attP integrated into intron 1 of the Gt(ROSA)26Sor locu. CaMPARI (Calcium Modulated Photoactivatable Ratiometric Integrator) is a photoconvertible protein construct derived from the stony coral protein Eos. CaMPARI and irreversibly photoconverts from green to red in the presence of violet light and high intracellular calcium. The CaMPARI protein construct includes a calcium binding calmodulin with a calmodulin/Ca<2+> binding peptide (M13) fused to either end of the protein making the photoconversion calcium dependent. Calcium binding affinity variants of the original CaMPARI (CaMPARI1) fluorescent protein were identified (CaMPARI1_W391F-V398L_Q142V-F198Y-C202T-L217I-N345S) and incorporated in CaMPARI2 via targeted mutagenesis of amino acids around the fluorescent protein chromophore and at the protein interface between the fluorescent protein and the calcium-sensitive domains.
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For Research Use Only.
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