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D2.Cg-Tg(Gfap-cre)73.12Mvs/SjJ
Cat. No.: CEMM-07250612
GFAP-Cre transgenic mice from founder line 73.12 have Cre recombinase expression directed by the mouse glial fibrillary acidic protein promoter. Cre expression is observed in astrocytes in the brain and spinal cord, as well as postnatal and adult GFAP-expressing neural stem cells and their progeny in the brain.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Tg(Gfap-cre)73.12Mvs
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| Allele Name |
transgene insertion 73.12
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| Allele Attributes |
Recombinase-expressing
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| Gene Symbol |
Tg(Gfap-cre)73.12Mvs
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| Gene Name |
transgene insertion 73.12
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| Chromosome |
UN
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| Expressed Genes |
Cre, Cre recombinase, bacteriophage P1
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| MGI Accession ID | show more close |
| Site of Expression |
Cre recombinase activity is observed in essentially all adult neural stem cells and their progeny in the hippocampal dentate gyrus and subventricular zone. In addition, some hippocampal pyramidal neurons and cerebellar granule neurons (less than 5%) and scattered neurons in the midbrain (less than 0.5%) also exhibit activity.
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| Strain of Origin |
(BALB/c x C57BL/6NHsd)F1
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| Molecular Note |
The bacterial Cre recombinase gene was inserted into the first exon of an expression cassette (clone 445) that contains all the introns, promoter regulatory elements, exons, and 2.5 kb of 5' and 2 kb of 3' flanking DNA of the astroglia-specific mouse glial fibrillary acidic protein gene. GFAP is not expressed from the transgene due to deletion of part of exon 1.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
DBA/2J Noncarrier
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| Breeding Considerations |
When maintaining a live colony, homozygous mice may be bred together. Of note, the donating investigator reports GFAP-Cre lines 73.12 and 77.6 have cre expression in the male germline, and suggests breeding GFAP-Cre females with floxed males for Cre-lox experiments.
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For Research Use Only.
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