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STOCK Igs2tm1(ACTB-EGFP, -tdTomato)Luo/J

Cat. No.: CEMM-07250282

Common Name: HIPP11GT
These intergenic site 2 (commonly referred to as MADM-11GT) targeted mutants are useful for generating fluorescent double markers in mice for studies to ascertain lineal relationships and pleiotropic gene function in multicellular organisms, and in studies of cell differentiation and mitosis.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Igs2tm1(ACTB-EGFP,-tdTomato)Luo
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Allele Name
targeted mutation 1
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Allele Attributes
Reporter; Null/Knockout
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Gene Symbol
Igs2
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Gene Name
intergenic site 2
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Chromosome
11
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Expressed Genes
GFP, Green Fluorescent Protein; RFP, Red Fluorescent Protein, coral
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MGI Accession ID
4837736
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Site of Expression
Cre recombination results in EGFP and tdTomato mosaic expression in Cre-expressing tissues When using the MADM-11 system.
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Strain of Origin
(129X1/SvJ x 129S1/Sv)F1-Kitl+
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Molecular Note
The GT MADM targeting construct was designed with a CMV enhancer/chicken beta-actin core promoter (pCA), the N-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP; aa 1-273), a beta-globin intronic sequence containing an FRT site and loxP-flanked neomycin resistance gene, the C-terminal portion of a red fluorescent protein (tdTomato; aa 4-1544) tagged with three copies of the Myc epitope at its C-terminus, and an SV40 T-antigen poly(A) signal. This entire GT MADM construct was inserted into the "Hipp11" locus on chromosome 11 (cytoband A1 at ~3cM between the Eif4enif1 and Drg1 loci).
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HUSBANDRY
Suggested Controls
C57BL/6J
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Breeding Considerations
When maintaining a live colony, homozygous mice may be bred together.
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For Research Use Only.
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