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B6N.129-Gt(ROSA)26Sortm2(CAG-KDM1A/EGFP)Rsd/J
Cat. No.: CEMM-07250898
Common Name: LSD1 VN
Cre-mediated excision of a floxed Stop in these LSD1 VN mice enables expression of FLAG-tagged human KDM1A (LSD1) cDNA encoding LSD1n isoforms of the gene, as well as an EGFP fluorescent reporter. These mice have been used for chromatin immunoprecipitation (ChIP) analysis.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm2(CAG-KDM1A/EGFP)Rsd
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| Allele Name |
targeted mutation 2
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Inserted expressed sequence; Humanized sequence; Modified isoform(s); Epitope tag
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
KDM1A, lysine demethylase 1A, human; GFP, Green Fluorescent Protein; FLAG, FLAG octapeptide
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| MGI Accession ID | show more close |
| Site of Expression |
Neurons
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| Strain of Origin |
(129X1/SvJ x 129S1/Sv)F1-Kitl+
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| Molecular Note |
The targeting vector contains: CAG-loxP-(PGK-neomycin - 4x polyA) Stop-loxP-biotin ligase recognition peptide (BLRP)-3x FLAG-LSD1 VN-IRES2-EGFP-4x polyA. The LSD1 VN (KDM1A) insertion expresses a FLAG-tagged full-length human KDM1A cDNA, which encodes an 872 amino acid isoform of the gene that includes a 12 nucleotide exon in intron 8 (E8a) expressed only in neurons as well as an EGFP fluorescent reporter.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6NJ
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| Breeding Considerations |
Heterozygotes and homozygotes are viable and fertile.
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| Breeding Strategy |
Heterozygote x Heterozygote
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For Research Use Only.
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