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B6N;129-Gt(ROSA)26Sortm1(CAG-GCaMP3)Dbe/J
Cat. No.: CEMM-07250698
Common Name: Rosa26-lsl-GCaMP3
Rosa26-lsl-GCaMP3 mice express the fluorescent calcium indicator protein GCaMP3 after exposure to Cre recombinase. Following subsequent calcium binding (such as neuronal activation), bright EGFP fluorescence is observed.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm1(CAG-GCaMP3)Dbe
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Reporter
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
GCaMP, Genetically encoded calcium indicator; GFP, Green Fluorescent Protein
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| MGI Accession ID | show more close |
| Site of Expression |
When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the Cre-expressing tissues; resulting in expression of the fluorescent calcium indicator protein, GCaMP3.
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| Strain of Origin |
129
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| Molecular Note |
The Rosa-CAG-LSL-GCaMP3-WPRE targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP-flanked STOP cassette, a GCaMP3 cDNA, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a BGH polyA signal, and an FRT flanked Neo cassette. The GCaMP3 fusion gene contains a cDNA sequence encoding the chicken smooth muscle M13 fragment of myosin light chain kinase, a circularly permutated EGFP (amino acid residues 149-238 followed by amino acid residues 1-144 (including M153K, V163A, S175G, D180Y, T203V, A206K, and V93I mutations to inCrease dynamic range and baseline fluorescence), and a DNA fragment encoding amino acid residues 2-148 of the rat calmodulin (modified to harbor the N60D mutation to inCrease the fluorescence change for small calcium transients).
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6NJ
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| Breeding Considerations |
When maintaining a live colony, homozygous mice may be bred together.
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| Breeding Strategy |
Heterozygote x Heterozygote
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For Research Use Only.
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