C57BL/6-Tg(Npas1-icre, -tdTomato)1Cschn/J

Cat. No.: CEMM-07250640

Common Name: Npas1-tdTom

These Npas1-tdTom mice express icre and tdTomato in most (approximately 88%) of Npas1-expressing neurons in the brain. They are suitable for use in studies related to the external globus pallidus (GPe) nucleus in the basal ganglia.

Inquiry

Status	Live Mouse Frozen Embryo
Age	4 weeks 12 weeks Customized Age
Sex	Male Female

GENETICS
Allele Symbol	Tg(Npas1-icre,-tdTomato)1Cschn show more close
Allele Name	transgene insertion 1 show more close
Allele Attributes	Recombinase-expressing; Reporter show more close
Gene Symbol	Tg(Npas1-icre,-tdTomato)1Cschn Tg(Npas1-icre,-tdTomato)1Cschn show more close
Gene Name	transgene insertion 1 show more close
Chromosome	UN show more close
Expressed Genes	Cre, Cre recombinase, bacteriophage P1; RFP, Red Fluorescent Protein, coral show more close
MGI Accession ID	5816528 show more close
Site of Expression	This transgene is expressed in neurons of the external globus pallidus nucleus in the basal ganglia, cortex, hippocampus, basal magnocellular complex, internal globus pallidus, zona incerta, substantia nigra pars reticulata, superior colliculus, and in some neurons in the dorsal striatum and thalamus. show more close
Strain of Origin	C57BL/6J show more close
Molecular Note	A mouse bacterial artificial chromosome (BAC) containing the entire Npas1 gene, was modified by inserting iCre-2A (cleavable fusion peptide sequence)-tdTomato and polyadenylation sequence from bovine growth hormone downstream of the ATG translation initiation codon, and removing Npas1 coding sequence. A transgenic insert made from this modified BAC was microinjected into the pronucleus of C57BL/6 fertilized eggs. The resulting Npas1-tdTom transgenic mice from founder line 1 were subsequently maintained on the C57BL/6J background. show more close

HUSBANDRY
Suggested Controls	C57BL/6J show more close
Breeding Considerations	When maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice. Homozygous viability/fertility has not been tested. show more close

For Research Use Only.