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B6N.129S6(B6)-Chattm2(cre)Lowl/J
Cat. No.: CEMM-07250411
Common Name: ChAT-IRES-Cre; ChAT-IRES-Cre::frt-neo-frt; B6N.ChAT-IRES-Cre; B6N.ChAT-IRES-Cre::frt-neo-frt
ChAT-IRES-Cre knock-in mice express Cre recombinase in cholinergic neurons, without disrupting endogenous Chat expression. These mice may be useful in neurobiological research of motor function, learning and memory, Alzheimer's disease, and Down syndrome, as well as obesity and diabetes research. our repository has two ChAT-IRES-Cre knock-in alleles from Dr. Bradford Lowell: ChAT-IRES-Cre::SV40pA::frt-neo-frt and ChAT-IRES-Cre::SV40pA::Δneo (Chattm1(cre)Lowl ;). Each allele also contains a partial neo fragment between the Cre and SV40 polyA. The donating investigator reports that the presence of the frt-flanked neo cassette may result in ectopic Cre expression, and they have never observed any ectopic expression in their ChAT-IRES-Cre::SV40pA::Δneo mice (January 2018).
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Chattm2(cre)Lowl
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| Allele Name |
targeted mutation 2
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| Allele Attributes |
Recombinase-expressing
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| Gene Symbol |
Chat
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| Gene Name |
choline acetyltransferase
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| Chromosome |
14
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| Expressed Genes |
Cre, Cre recombinase, bacteriophage P1
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| MGI Accession ID | show more close |
| Site of Expression |
Cre recombinase activity is reported in all cholinergic neurons.
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| Strain of Origin |
129S6/SvEvTac
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| Molecular Note |
An optimized internal ribosome entry sequence (IRES) fused to Cre recombinase followed by a neomycin resistance cassette flanked FRT sites was inserted after the ChAT stop codon in BAC (bMQ185k21) using homologous recombination in bacteria. A genomic ChAT fragment with 4kb homology arms on either side of the IRES-Cre insertion site was lifted out from the BAC using reverse recombineering techniques and transformed into ES cells. This allele was used to generate Chattm1(Cre)Lowl.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6NJ
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| Breeding Considerations |
Mice were bred to C57BL/6NJ inbred mice for many generations using a marker-assisted, speed congenic approach to generate this C57BL/6NJ-congenic strain. When maintaining the live congenic colony, homozygous mice may be bred together.
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| Breeding Strategy |
Homozygote x Homozygote
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For Research Use Only.
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