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STOCK Gt(ROSA)26Sortm1(CAG-cas9*, -BFP)Khk/Mmjax
Cat. No.: CEMM-07250893
Removal of this mouse colony is imminent. If live mice are needed for your studies, it is advised that they be ordered immediately. After removal, the mice will be available from a cryorecovery.Rosa26-LSL-dCas9 mice (Gt(ROSA)26Sortm1(CAG-dCas9-SunTag)Khk) are a Cre-inducible CRISPR/Cas9 activation (CRISPRa) tool for cell type specific gene activation in vivo.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm1(CAG-cas9*,-BFP)Khk
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Epitope tag; Endonuclease
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
CFP, Cyan Fluorescent Protein, jellyfish; cas9, CRISPR associated protein 9
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| MGI Accession ID | show more close |
| Site of Expression |
After exposure to Cre recombinase to remove the STOP cassette, dCas9/SunTag is expressed in Cre expressing tissues.
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| Strain of Origin |
(C57BL/6 x 129S4/SvJae)F1
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| Molecular Note |
The pAi9-dCas9 targeting construct introduced into the locus a CAG promoter, floxed stop cassette, neomycin selection cassette, a nuclease dead Cas9 sequence tagged with 10 copies of the GCN4 peptide v4 (SUperNova; SunTag), 2A sequence and BFP reporter gene.
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| HUSBANDRY | |
|---|---|
| Breeding Considerations |
When maintaining a live colony, these mice are bred by homozygote sibling matings.
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| Breeding Strategy |
Homozygote x Homozygote
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For Research Use Only.
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