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C57BL/6-Gt(ROSA)26Sortm8(Map2k1*, EGFP)Rsky/J
Cat. No.: CEMM-07250218
The R26StopFLMEK1DD conditional allele is targeted to the Gt(ROSA)26Sor locus and has a loxP-flanked Neo-STOP cassette preventing transcription of the downstream bicistronic sequences (MEK1DD [a mutant form of rat MAPKK1 rendered constitutively active by two serine->aspartic acid substitutions (S218D/S222D) within the catalytic domain] and EGFP). These R26StopFLMEK1DD mice allow inducible constitutive activity of MAP Kinase signal transduction pathways that mediate various cellular activities, including gene expression, mitosis, differentiation, proliferation, cell survival, and cell cycle progression.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm8(Map2k1*,EGFP)Rsky
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| Allele Name |
targeted mutation 8
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Constitutively active; Inserted expressed sequence
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
Map2k1, mitogen activated protein kinase kinase 1, rat; GFP, Green Fluorescent Protein
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| MGI Accession ID | show more close |
| Site of Expression |
Cre excision of the stop signal results in expression of EGFP and a constitutively active form of rat MAPKK1 (MEK1DD) in Cre-expressing tissues.
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| Strain of Origin |
C57BL/6
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| Molecular Note |
A targeting vector designed with (from 5' to 3') a splice acceptor site, a loxP-flanked Neo-STOP cassette, a cDNA sequence encoding rat MEK1DD (a mutant form of MAPKK1 rendered constitutively active by two serine to aspartic acid substitutions (S218D/S222D) within the catalytic domain], an FRT flanked internal ribosomal entry site-enhanced green fluorescent protein cassette (IRES-EGFP), and bovine polyadenylation signal. This transgene construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
When maintaining a live colony, homozygous mice may be bred together.
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| Breeding Strategy |
Homozygote x Homozygote
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For Research Use Only.
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