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C57BL/6-Gt(ROSA)26Sortm1(CAG-GFP, -Mars*L274G)Esm/J
Cat. No.: CEMM-07250653
Common Name: FUNCAT
This STOPflox R26-MetRS* knock-in strain enables genetically targeted (Cre-dependent) metabolic labeling of endogenous proteins in specific cell-types in vivo for subsequent imaging or purification and biochemical analysis.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm1(CAG-GFP,-Mars*L274G)Esm
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Inserted expressed sequence
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
GFP, Green Fluorescent Protein; Mars1, methionine-tRNA synthetase 1, mouse, laboratory
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| MGI Accession ID | show more close |
| Site of Expression |
Following Cre recombinase mediated removal of the floxed STOP cassette, GFP expression and ANL incorportation are only detected in cells expressing the mutant methionine-tRNA synthetase, MetRS* (Mars*).
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| Strain of Origin |
C57BL/6NTac
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| Molecular Note |
The exchange vector is designed to contain a mutant F3 FRT site, a NEO selection cassette, CAG promoter, a floxed STOP cassette, Green Fluorescent Protein coding sequence, foot-and-mouth disease virus 2A peptide sequence (to mediate ribosomal skipping), the mouse methionine-tRNA synthetase (Mars) with the L274G point mutation, and an FRT site. This vector was integrated into the Gt(ROSA)26Sor locus by recombinase mediated cassette exchange (RMCE). The L274G mutation allows the binding of the methionine surrogate azidonorleucine (ANL) onto the methionine tRNA. ANL can be tagged for imaging or affinity purification.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
When maintaining a live colony, these mice can be bred as homozygotes.
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| Breeding Strategy |
Homozygote x Homozygote
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For Research Use Only.
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