B6.FVB-Tg(CAG-EGFP, -Sumo1, -Sumo2, -Sumo3, -mCherry)10Weiy/J

Cat. No.: CEMM-07250885

Common Name: CAG-SUMO line 10

CAG-SUMO line 10 mice have widespread EGFP fluorescence that can be replaced by SUMO1/2/3 paralog expression and mCherry fluorescence after Cre recombinase exposure. These mice may be useful for studying in vivo SUMO proteomics - for example, mechanistically linking SUMO conjugation (SUMOylation) and the SUMO-modified proteome to the pathological processes of human disorders.

Inquiry

Status	Live Mouse Frozen Embryo
Age	4 weeks 12 weeks Customized Age
Sex	Male Female

GENETICS
Allele Symbol	Tg(CAG-EGFP,-Sumo1,-Sumo2,-Sumo3,-mCherry)10Weiy show more close
Allele Name	transgene insertion 10 show more close
Allele Attributes	Conditional ready (e.g. floxed); Reporter; Inserted expressed sequence; Epitope tag show more close
Gene Symbol	Tg(CAG-EGFP,-Sumo)10Weiy show more close
Gene Name	transgene insertion 10 show more close
Chromosome	UN show more close
Expressed Genes	GFP, Green Fluorescent Protein; RFP, Red Fluorescent Protein, coral show more close
MGI Accession ID	6150883 show more close
Site of Expression	In the absence of Cre recombinase, the donating investigator reports widespread, but dim, EGFP expression is observed in all organs examined (including brain, heart, lung, kidney and liver). Following Cre-mediated deletion of the floxed-EGFP-STOP cassette, robust mCherry fluorescence is seen in Cre-expressing tissues. show more close
Strain of Origin	FVB/N show more close
Molecular Note	The transgene was designed to have (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP site, EGFP, a transcriptional/translational STOP cassette (containing a PGK-neo), a second loxP site, a mouse cDNA sequence encoding precursor SUMO1 with N-terminal histidine tag (His-SUMO1), a viral 2A oligopeptide sequence (P2A; mediates ribosomal skipping), a mouse cDNA sequence encoding precursor SUMO2 with N-terminal hemagglutinin tag (HA-SUMO2), a viral 2A oligopeptide sequence (T2A; mediates ribosomal skipping), a mouse cDNA sequence encoding precursor SUMO3 with N-terminal FLAG tag (FLAG-SUMO3), a viral 2A oligopeptide sequence (F2A; mediates ribosomal skipping), the monomeric DsRed fluorescent protein variant mCherry gene and a bovine growth hormone polyadenylation signal. Eight founder lines were Created, founder line 10 exhibits ubiquitous expression of GFP. show more close

HUSBANDRY
Suggested Controls	C57BL/6J show more close
Breeding Considerations	When maintaining a live colony, hemizygous mice may be bred together, to wildtype (non-carrier) mice from the colony or to C57BL/6J inbred mice. the phenotype of homozygous mice has not been characterized. show more close
Breeding Strategy	Noncarrier x Hemizygote; Hemizygote x Noncarrier show more close

For Research Use Only.