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STOCK Gt(ROSA)26Sortm3.1(CAG-HRP)Ddg/J
Cat. No.: CEMM-07250996
Common Name: ROSA26LSL-ER-HRP
ROSA26LSL-ER-HRP mice conditionally express endoplasmic reticulum (ER)-targeted horse radish peroxidase (HRP) in a Cre recombinase-dependent manner. Cre recombinase-mediated excision of a loxP-flanked Stop cassette enables directed expression of horse radish peroxidase fused to an endoplasmic reticulum retention signal, useful in multiplexed electron microscopy labeling. The donating laboratory describes this strain as an ER-ERP strain: horseradish peroxidase fused to immunoglobulin kappa light chain leader sequence and KDEL endoplasmic reticulum retention signal.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm3.1(CAG-HRP)Ddg
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| Allele Name |
targeted mutation 3.1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| MGI Accession ID | show more close |
| Strain of Origin |
129S4/SvJae
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| Molecular Note |
The targeting vector is Created by replacing the tdTomato cassette in Gt(ROSA)26Sortm65.1(CAG-tdTomato)Hze with DR-ER-HRP, a dual (Cre and Flp) recombinase (DR)-dependent, endoplasmic reticulum (ER)-targeted, horse radish peroxidase (HRP) reporter cassette. The following was introduced to intron 1 of the mouse Gt(ROSA)26Sor gene - CAG-FRT Stop-FRT loxP-Stop-loxP-ER ("KDEL" target peptide for endoplasmic reticulum retention)-HRP (horse radish peroxidase)-WPRE (Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element)-bGHpA (bovine growth hormone polyadenylation signal)-attB-PGK-NeoR-PGKpA-attP. Flp-mediated recombination removed the FRT flanked stop cassette.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
Wild-type from the colony
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| Breeding Considerations |
Heterozygotes and homozygotes are viable and fertile.
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| Breeding Strategy |
Heterozygote x Heterozygote
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For Research Use Only.
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