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C.129S(B6)-Chrna3tm1.1Hwrt/J

Cat. No.: CEMM-07250231

These α3[5] mice contain a mutation designed to introduce 5 nucleotide mutations to exon 5 of mouse nicotinic acetylcholine receptor (nAChR) neuronal α3 subunit and a loxP-flanked neomycin (neo) resistance cassette upstream of exon 5. These mice may be useful for studying the pathological mechanisms of receptor assembly and trafficking in neurological and psychiatric disorders including Alzheimer's disease, Parkinson's disease, schizophrenia, and epilepsy, as well as drug addiction, response to pain, and nicotine induced behaviors.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Chrna3tm1.1Hwrt
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Allele Name
targeted mutation 1.1
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Allele Attributes
Not Specified
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Gene Symbol
Chrna3
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Gene Name
cholinergic receptor
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Chromosome
9
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MGI Accession ID
4442209
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Strain of Origin
129S7/SvEvBrd-Hprtb-m2
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Molecular Note
A targeting construct was made containing a mutated exon 5 of mouse nicotinic acetylcholine receptor (nAChR) neuronal alpha3 subunit (alpha3[5]), and a loxP-flanked neomycin (neo) resistance cassette upstream of exon 5. This mutated alpha3[5] gene contained five altered nucleotides, Y184W, E187W, I188V, K189Y, and N191T, in the loop C region corresponding to residues from the alpha1 subunit of nAchR from Torpedo californica, the Pacific electric ray which conveys alpha-bungarotoxin (alphaBTX) sensitivity. Cre mediated recombination removed the neo cassette.
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HUSBANDRY
Suggested Controls
BALB/cJ Wild-type from the colony
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Breeding Considerations
When maintaining a live colony heterozygous mice may be bred to wildtype mice from the colony or BALB/cJ inbred mice. Homozygous mice are infertile.
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For Research Use Only.
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