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129S-H3f3atm1.1Mnn/J
Cat. No.: CEMM-07250459
These H3f3ac/c mutant mice possess loxP sites flanking a wildtype H3 histone isoform 3 (H3.3) coding sequence and STOP sequence. When bred to mice expressing cre-recombinase, removal of the floxed sequence results in YFP expression. This strain may be useful for studying the role of histone H3.3 during development.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
H3f3atm1.1Mnn
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| Allele Name |
targeted mutation 1.1
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| Allele Attributes |
Conditional ready (e.g. floxed); No functional change
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| Gene Symbol |
H3f3a
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| Gene Name |
H3 histone
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| Chromosome |
1
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| MGI Accession ID | show more close |
| Strain of Origin |
129S1/SvImJ
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| Molecular Note |
A targeting vector was designed to contain, from 5' to 3', a synthetic splice acceptor, a loxP site, an ATG start codon, a wild-type H3 histone isoform 3 (H3.3) coding sequence, H3f3a (H3 histone, family 3A) 3' UTR, and an SV40 polyadenylation (polyA) sequence. This was followed by a downstream FRT flanked neomycin resistance (neo) cassette, and a second loxP site, an ATG start codon, the venus variant of yellow fluorescent protein (YFP), and another SV40 polyA sequence. This vector replaced part of exon 2. Flp-mediated recombination removed the neo cassette.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
129S1/SvImJ
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| Breeding Considerations |
When maintaining a live colony, homozygous mice may be bred together.
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For Research Use Only.
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